Localization of the Succinic Dehydrogenase System in Escherichia Coli Using Combined Techniques of Cytochemistry and Electron Microscopy
نویسندگان
چکیده
The activity of the succinic dehydrogenase system was studied in Escherichia coli utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by the deposition of aggregates of TNBT-formazan, was found associated with the site of the plasma membrane of the bacterium.
منابع مشابه
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Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytop...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 24 شماره
صفحات -
تاریخ انتشار 1965